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RUVBL2 promotes tumorigenesis in SKCM. A Representative IHC images showing FOXM1, NOX4, and NOTCH3 expression in SKCM versus normal skin tissues. B Relative mRNA levels in normal human epidermal <t>melanocytes</t> (MC) and SKCM cell lines. C RUVBL2 mRNA expression in A375 and SK-Mel-5 cells following transfection with si-RUVBL2 versus control. D CCK-8 cell viability assay in A375 and SK-Mel-5 cells after si-RUVBL2 knockdown. E EdU incorporation assay showing proliferation of A375 and SK-Mel-5 cells post–si-RUVBL2 transfection. * p < 0.05, ** p < 0.01, *** p < 0.001
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RUVBL2 promotes tumorigenesis in SKCM. A Representative IHC images showing FOXM1, NOX4, and NOTCH3 expression in SKCM versus normal skin tissues. B Relative mRNA levels in normal human epidermal <t>melanocytes</t> (MC) and SKCM cell lines. C RUVBL2 mRNA expression in A375 and SK-Mel-5 cells following transfection with si-RUVBL2 versus control. D CCK-8 cell viability assay in A375 and SK-Mel-5 cells after si-RUVBL2 knockdown. E EdU incorporation assay showing proliferation of A375 and SK-Mel-5 cells post–si-RUVBL2 transfection. * p < 0.05, ** p < 0.01, *** p < 0.001
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RUVBL2 promotes tumorigenesis in SKCM. A Representative IHC images showing FOXM1, NOX4, and NOTCH3 expression in SKCM versus normal skin tissues. B Relative mRNA levels in normal human epidermal <t>melanocytes</t> (MC) and SKCM cell lines. C RUVBL2 mRNA expression in A375 and SK-Mel-5 cells following transfection with si-RUVBL2 versus control. D CCK-8 cell viability assay in A375 and SK-Mel-5 cells after si-RUVBL2 knockdown. E EdU incorporation assay showing proliferation of A375 and SK-Mel-5 cells post–si-RUVBL2 transfection. * p < 0.05, ** p < 0.01, *** p < 0.001
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RUVBL2 promotes tumorigenesis in SKCM. A Representative IHC images showing FOXM1, NOX4, and NOTCH3 expression in SKCM versus normal skin tissues. B Relative mRNA levels in normal human epidermal melanocytes (MC) and SKCM cell lines. C RUVBL2 mRNA expression in A375 and SK-Mel-5 cells following transfection with si-RUVBL2 versus control. D CCK-8 cell viability assay in A375 and SK-Mel-5 cells after si-RUVBL2 knockdown. E EdU incorporation assay showing proliferation of A375 and SK-Mel-5 cells post–si-RUVBL2 transfection. * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Discover Oncology

Article Title: Cellular senescence related gene signature predicts prognosis and immune features in skin cutaneous melanoma

doi: 10.1007/s12672-025-03994-y

Figure Lengend Snippet: RUVBL2 promotes tumorigenesis in SKCM. A Representative IHC images showing FOXM1, NOX4, and NOTCH3 expression in SKCM versus normal skin tissues. B Relative mRNA levels in normal human epidermal melanocytes (MC) and SKCM cell lines. C RUVBL2 mRNA expression in A375 and SK-Mel-5 cells following transfection with si-RUVBL2 versus control. D CCK-8 cell viability assay in A375 and SK-Mel-5 cells after si-RUVBL2 knockdown. E EdU incorporation assay showing proliferation of A375 and SK-Mel-5 cells post–si-RUVBL2 transfection. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Normal human epidermal melanocytes and melanoma cell lines (A375 and SK-Mel-5) were obtained from the American Type Culture Collection (ATCC) and cultured under standard conditions as previously described [ ].

Techniques: Expressing, Transfection, Control, CCK-8 Assay, Viability Assay, Knockdown